The collection comprises manuscript notebooks, printed pamphlets, correspondence, a minute book, and photographic and other catalogues, 1768-1970. These notably include notebooks containing descriptions and viewing data relating to astronomical observations carried out at Kew Observatory, Richmond, Surrey, on behalf of King George III, with a printed pamphlet by Nevil Maskelyne, Astronomer Royal, entitled Observations of the transit of Venus over the Sun , 1768-1769; manuscript notebook containing measurements recorded during a test of the accuracy of the H5 chronometer designed by John Harrison, 1772; manuscript notebooks recording daily temperature, barometric, hygrometric and rainfall readings taken at Kew Observatory, Richmond, Surrey, 1773-1840; a printed pamphlet by George Atwood, Fellow of the Royal Society, entitled A Supplement to 'A treatise on the construction and properties of arches' (London, 1804), with manuscript dedication to King George III and related correspondence, 1804-1805; a folio signature book of visits by dignitaries to the George III Museum, 1843-1929; manuscript diary describing the work of the George III Museum including the upkeep of equipment and use of exhibits in experiments, 1850-1880; manuscript minutes of the George III Museum Committee charged with managing the collection, 1880-1885; catalogues relating to the collection, [1841-1970]; correspondence by George Calver, astronomer, relating to telescope design, 1897; series of copper plates and labels advertising the George III Museum, [1841-1926].

Experimental notes, working papers, correspondence and lecture summaries compiled by Charles Wheatstone, 1836-1875, and photographs collected by him in that period. Notably including papers relating to the development and testing of the telegraph, [1836-1960]; descriptions of experiments and test results concerning the measurement of electromotive forces and electrical potential, [1840-1875]; experimental observations on the nature of magnetism, electricity and thermodynamics, including electromagnet design, batteries and dynamos, [1834-1855]; working papers relating to optics including experiments into refraction, colouration of compounds and polarisation, [1850-1875]; drafts of lectures on sound and musical instruments prepared by Wheatstone, [1832-1837]; material relating to the management of the Wheatstone collection of scientific instruments and library, 1890-1992; biographical material relating to the life of Wheatstone, the invention of the telegraph and Wheatstone's musical instrument manufacturing business, with unrelated newspapers, 1757-1975; stereoscopic photographs and glass negatives taken by Roger Fenton, Samuel Buckle, Jules Duboscq and others, featuring landscapes, still lifes, panoramic scenes of cities including Paris and Moscow and the interior and exterior of the Crystal Palace, Hyde Park, Sydenham, 1851, and especially the Exposition Universelle, Paris, 1855, [1850-1901]; artefacts from Department of Physics, King's College London, including demonstration equipment, telegraph apparatus, a nail fiddle and other prototype musical instruments, [1834-1875]; exhibition of scientific and musical instruments, [1834-1875].